Recombinant IL2R gamma C anticorps

N° du produit ABIN7072572

Cet anticorps anti- est un anticorps Rat Monoclonal détectant dans BP, FACS et IP. Adapté pour Souris.

Aperçu rapide pour Recombinant IL2R gamma C anticorps (ABIN7072572)

Antigène: IL2R gamma C

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Blocking Peptide (BP), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 3E12

Détail du produit

Fonction: Anti-IL2R gamma C [3E12], Rat IgG2b, kappa

Specificité: This conformation-dependent antibody binds to the murine common gamma-chain, shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptor complexes. It recognises an epitope located in a membrane-proximal portion of the common gamma-chain, near Arg-197 of domain 2. This antibody does not cross-react with COS7 cells transfected with rat common gamma-chain cDNA (Olosz et al, 2002).

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing murine cytoplasmic-tailless IL-2R common gamma-chain (YB2/0-myt).

Isotype: IgG2b kappa

Information d'application

Indications d'application: This antibody has been used in flow cytometric analysis of common gamma-chain surface expression by IL-7-stimulated T cells (Goh et al, 2017) and COS7 monkey kidney cells (Olosz et al, 2002). This antibody has also been used to immunoprecipiate murine common gamma-chain-containing complexes from COS7 cell extracts (Olosz et al, 2002). Additionally, as this antibody is conformationally sensitive, it has been used to confirm the correct folding of soluble murine common gamma chain mutants (Meißner et al, 2001). This antagonistic antibody is most effective at inhibiting IL-4 bioactivity (Malek et al, 1998). Treatment of recipient mice with this antibody, along with another common gamma-chain-blocking MAb (4G3), significantly prolongs islet allograft survival (Li et al, 2000). Indeed, blocking the common gamma chain with this antibody has been shown to induce apoptotic cell death of BAF-B03 cells and primary anti-CD3 activated T lymphoblasts in vitro, and of alloreactive T cells in vivo (Li et al, 2000).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: IL2R gamma C

Sujet: CD132, IL2R gamma C, common gamma chain, common y chain, gamma chain, IL-2 receptor gamma chain, Interleukin-2 receptor subunit gamma, Cytokine receptor common subunit gamma, Il2rg, IL-2 receptor subunit gamma, IL-2R subunit gamma, IL-2RG, gammaC, p64

UniProt: P34902